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The third, and least energetically favorable, binding pose obtained for CYP3A7 oriented the testosterone substrate in position for β-hydroxylation at the C2 atom (Fig. 7E). Interestingly, the 2α-OH-T/6β-OH-T metabolite ratio calculated for CYP3A7 was ≥1 at low concentrations of testosterone; however, it decreased to β-OH-T/6β-OH-T metabolite ratio for the three CYP3A enzymes remained constant throughout the entire substrate concentration range tested. In summary, here we have demonstrated that the metabolite profile of testosterone oxidation by CYP3A7 is substantially distinct from that of CYP3A4/5 and that the type and concentration of metabolites produced varies with substrate concentration, which likely is due to homotropic cooperativity. Julia Walker, RN, BSN, is a clinical nurse specializing in helping patients with thyroid disorders. Before making changes to your diet, it is necessary to consult your thyroid doctor. 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