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Previous research using a variety of procedures has demonstrated that participation in competition increases testosterone levels, and these increases tend to be greater in winners than in losers (for a review see Archer 2006). Crucially, then, definitive evidence of an association between circulating testosterone and facial masculinization in adult men has yet to be found, even though a large literature on human facial preferences is based on this assumption (see Rhodes 2006 for a review). In men, testosterone levels are affected by the outcome of competition, increasing in winners relative to losers in physical (Mazur & Lamb 1990) and non-physical (Gladue et al. 1989; Mazur et al. 1992; McCaul et al. 1992) contests. Consistent with the ‘challenge hypothesis’ (Wingfield et al. 1990) men respond to competitive situations with increased testosterone levels (for a review see Archer 2006). 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