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Effects On Male Testosterone Levels A Comprehensive Approach to Low Testosterone and Fertility Q：Male Reproductive System A Level and IB BiologyA：Short GC columns (e.g., 5–10 m 0.25 mm i.d.) with a small amount of stationary phase are usually preferred to speed up analysis time and to minimize baseline drift.135 Recently, new stabilized non-polar columns operating up to 400 C (which allows hydrocarbon elution up to C80) have been developed. After a certain time, which depends on the volume of the sample introduced, the split line is closed and the PTV is heated up, transferring the components from the injector to the analytical column. When using classic GC-FID analysis (off-line), the on-column injection technique is to be preferred to prevent losses of volatile hydrocarbons and discrimination effects. 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