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In conclusion, our present study demonstrated that HTSCs might be putative SLCs in the testis and presented the development of a safe, rapid, and efficient strategy for the direct differentiation of hESCs into LLCs. To further specify LLCs, cells were treated with D + F + F2 medium containing PDGF-BB, LH, thyroid hormone, IGF-I, forskolin, FGF2 for 4 days on the IM. Any course of treatment referenced should not be followed or taken without consulting a physician, dentist or other health care provider. Further, references or links (provided by the ADA or by a user or guest blogger) to products or services do not constitute endorsements by the ADA. The ADA’s official opinions and policies can be found in its formal publications and websites (ADA.org and mouthhealthy.org). The ADA is the leading source of oral health-related information for dentists and their patients. 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