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Q：Testosterone Booster : Science and Benefits 2025A：However, the reason why there is a strong debate on this topic is probably that age-related comorbidities, including endocrinology and cardiovascular pathologies, can independently influence vitamin D assessment and circulating testosterone levels. Furthermore, one study claimed a negative association with free testosterone , while two studies reported a relationship with SHBG [73,77]. Indeed, some studies not showed a relationship between circulating 25-hydroxyvitamin D3 and total testosterone, free testosterone or SHBG [69,70,71], while others stated a positive association with total or free testosterone [72,73,74,75,76,77]. Furthermore, some of these studies revealed a significantly relationship between 25-hydroxyvitamin D3 and sex hormone-binding globulin (SHBG) [59,60,61,65]. Furthermore, possible bias, including age, body mass index, and baseline vitamin D status justified the differences among studies. 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